Aim of this work: To study and to evaluate how the Low Level Laser (LLL) irradiation has influence on human sperm motility, and the velocity of human spermatozoa which was calculated by means of multiple exposure photography. Methods: 50 semen samples were chosen from men of infertility; the volunteers were of age of 25-32-year. Semen samples were stored in formalin-saline for evaluation of concentration and sperm morphology, sperm concentration greater than 200 × 106/mL, and ejaculate volume greater than 5.0 ml. Samples were divided into five groups: a control group without laser irradiation, the other four samples groups were irradiated by an aluminum gallium indium phosphide laser diode with a wavelength of 910 nm, with 10, 12,14,18,20 min respectively, all samples were subjected for analysis using computer-assisted sperm analyzer.
Results: It was noticed that and after each dose of laser irradiation, sperm velocity and mobility were increased. Conclusion: Increasing of sperm velocity was due to Laser light stimulates non-motile live spermatozoa. Keywords: Laser, Human sperm, motility, fertilization