Diagnosis techniques of protein analysis using mass

Diagnosis of PCG is largelyclinical, and the molecular mechanisms underlying the responsible geneticdefects are not clearly understood so far. The concept of biological markers,many of which are proteins, has facilitated and improved the accuracy of diseasediagnosis in recent years as well as directing treatment regimens in some cases(Pandey A, Mann M. Proteomics to study genes and genomes. Nature.

2000 Jun15;405(6788):837-46.). Indeed, relative quantification of protein expressionhas been made possible through gel-based and gel-free techniques of proteinanalysis using mass spectrometry and isotope labeling-based or label-freetechniques. The aim of the current study was to characterise the proteincontent of AH in eyes with PCG.

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A preliminary report is presented herein. Thestudy identified 109 protein peaks of which 63 % were different between PCGpatients and controls. There have been published reports about the proteincontent of AH in primary open angle glaucoma, uveitic glaucoma and myopia (Grus,F.

H., Joachim, S.C., Sandmann, S., Thiel, U., Bruns, K., Lackner, K.J.

,Pfeiffer, N.,2008.Transthyretin and complex protein pattern in aqueous humor ofpatients with primary open-angle glaucoma. Mol. Vis.

14, 1437e1445., Ladas,J.G., Yu, F.

, Loo, R., Davis, J.L., Coleman, A.L.

, Levinson, R.D., Holland,G.N., 2001.

Relationship between aqueous humor protein level and outflowfacility in patients with uveitis. Invest. Ophthalmol. Vis. Sci. 42,2584e2588.

, Mackay, E.O., Kallberg, M.E.

, Gelatt, K.N., 2008. Aqueous humormyocilin protein levels in normal, genetic carriers, and glaucoma Beagles.

Vet.Ophthalmol. 11, 177e185.

, Duan, X., Lu, Q., Xue, P., Zhang, H., Dong, Z., Yang,F., Wang, N., 2008.

Proteomic analysis of aqueous humor from patients withmyopia. Mol. Vis. 14, 370e377., Kliuchnikova AA, Samokhina NI, Ilina IY, KarpovDS, Pyatnitskiy MA, Kuznetsova KG, Toropygin IY, Kochergin SA, Alekseev IB,Zgoda VG, Archakov AI, Moshkovskii SA. Human aqueous humor proteome incataract, glaucoma, and pseudoexfoliation syndrome.

Proteomics. 2016Jul;16(13):1938-46. doi: 10.

1002/pmic.201500423.). Bouhenni et al (Bouhenni RA, Al Shahwan S, Morales J, Wakim BT, Chomyk AM, Alkuraya FS, Edward DP. Identification of differentially expressedproteins in the aqueous humor of primary congenital glaucoma. Exp Eye Res. 2011 Jan;92(1):67-75.

doi:10.1016/j.exer.

2010.11.004. Epub 2010 Nov 13.

) attempted to identify proteins that may be altered in PCG.